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Hair Restoration
Hair Restoration | Protocol Guide
Hair Loss · Thinning · Scalp Coverage
This protocol combines an intramuscular scalp neuromodulator injection with RF microneedling and topical exosome application to support hair restoration over the loss area. The neuromodulator is injected once per cycle (its effect lasts about 4 months), while RF microneedling and exosome are repeated on a shorter interval to drive ongoing stimulation and delivery.
Why a Multimodality Approach: This protocol is designed to address all the layers involved in skin and scalp regeneration. The causes of hair loss are multifactorial - hormonal, vascular, inflammatory, nutritional, and follicular - so no single agent addresses every contributor. A multimodality, multi-layer approach is appreciated, and combination therapy is encouraged for the best outcomes.
How each component contributes to the regenerative layers:
Standard Reconstitution
Effect Duration: The neuromodulator effect lasts about 4 months, so the scalp injection is performed once during the first treatment of the cycle - not repeated at every session.
Deliver the neuromodulator intramuscularly across the scalp, working systematically to cover the full hair loss area.
Needle Options
32 G 6 mm · or 34 G 4 mm
Injection Angle
30-45° to the scalp
Volume Per Injection
0.5 cc each point
Total Injections
≤ 50 cover loss area
Spacing
1 cm intervals
Coverage Mapping: The right-to-left, crown-to-front pattern at 1-cm spacing ensures even, systematic coverage so no region of the loss area is missed.
During the first treatment, the neuromodulator injection is followed by RF microneedling and then topical exosome application, in this order:
First Treatment - Full Sequence
RF Microneedling Settings (SylfirmX)
After the first treatment, repeat RF microneedling and topical exosome (without re-injecting the neuromodulator) on a 4-week interval.
Maintenance - Repeat Sequence
Interval
4 weeks
Additional Sessions
2-4 more times
Cycle Logic: Because the neuromodulator lasts about 4 months, a single injection covers the cycle while the repeated RF microneedling + exosome sessions maintain stimulation and delivery every 4 weeks. Consistency is the key.
Because hair loss is multifactorial, this protocol pairs well with additional modalities that target different contributors. Combination therapy is encouraged and can be layered according to the patient's needs and your scope of practice:
Multi-Layer Principle: This protocol is built to address every layer of scalp and skin regeneration - muscle, dermis, vasculature, and follicle. Layering complementary modalities targets the multiple causes of hair loss simultaneously for stronger, more durable results.
Disclaimer: Educational reference for trained, licensed injectors. Confirm product indications, device settings, and local regulations before treatment. Device parameters should be adjusted to the individual patient and manufacturer guidance.
Yes, our products are thoughtfully formulated for all skin types.
Yes, our products are thoughtfully formulated for all skin types.
Yes, our products are thoughtfully formulated for all skin types.
SKIN BOOSTERCOCKTAILS
Skin Booster Cocktails | Master Protocol Guide
Master Protocol Guide for Skin Regeneration
Formulas · Dilutions · Mixing Protocols
Four Protocols
Foundations: Skin Botox Dilution
1. Glow Like Glass - fine lines, wrinkles, pores, texture
2. Glow from Within - laxity, sagging, loss of elasticity
3. Acne Calm & Brightening - acne, scars, pigment, dullness
Disclaimer: This guide is an educational reference for trained, licensed aesthetic injectors. Confirm product indications and local regulations before treatment. Injectable use of skin boosters is restricted in some regions, including the US.
Skin botox is delivered intradermally to improve skin texture, pore size, and sebum control. The two dilution methods below are referenced by every cocktail in this guide, so they are defined once here.
Standalone Dose
50-100 units
Combined With Boosters
20-40 units
Standard Reconstitution
Scenario A - Standalone: hyperdilute 1:1 with NaCl before injection.
50-Unit Hyperdiluted Formula
100-Unit Version: Aspirate 2.5 cc neurotoxin + 2.5 cc NaCl -> 5 cc total. Same 1:1 ratio applies.
Scenario B - Cocktail: skip the NaCl. The booster solution provides the volume, so aspirate neurotoxin directly into the booster.
20-40 Unit Cocktail Formula
Compatible boosters: HA · PN · PRP · PRF · Peptides · Exosomes · Elastic Labs
Dosing Tip: Adjust neurotoxin (20-40 units) proportionally to total booster volume - more volume, use the higher end of the range.
Reconstitute the entire 100-unit vial with a larger volume of NaCl for a ready-to-use solution in one step. This is the prep used by the cocktails when intradermal skin botox is needed.
Lower End - More Concentrated
Upper End - More Diluted
| DILUTION | TOTAL VOLUME | CONCENTRATION |
|---|---|---|
| 4 ml NaCl | 4 ml | 25 u / ml |
| 8 ml NaCl | 8 ml | 12.5 u / ml |
Aspiration Guide (20 units): Draw 0.8 cc from the 4 ml vial (25 u/ml) · Draw 1.6 cc from the 8 ml vial (12.5 u/ml).
Key Principle: Method 2 is for skin botox treatment only - do not use this preparation for cocktail mixing. Use 4 ml for a concentrated effect, 8 ml for larger, spreadable coverage.
These intradermal techniques apply across the cocktails in this guide. The goal in every case is shallow, even placement that raises a tiny skin elevation without the solution leaking.
Manual Needle Technique
Needle
34 G 2 mm or 4 mm
Angle
15° intradermal
Spacing
1 cm intervals
Microneedle Injector (e.g. Dermashine)
Using a microneedle injector device, deliver the skin botox or other skin booster solution directly into the skin. The device places the solution evenly across the treatment area.
Depth Check: Needle depth is appropriate when you see tiny skin elevations and the solution does not leak - or leaks minimally. The amount of acceptable leakage depends on the viscosity of the solution.
SkinVive (cross-linked HA) mixed with skin botox, delivered intradermally. Each SkinVive syringe is prepared and injected separately.
SkinVive Kit
2 syringes x 1 cc
Botox Per Syringe
20 units
Total Per Session
40 units
Key Point: Each 1 cc SkinVive syringe is mixed with 20 units of skin botox individually. The two syringes are not combined.
Prepare the neurotoxin using Method 2 (see Foundations). Aspirate 20 units and mix directly into each SkinVive syringe - no extra NaCl needed, the HA provides the volume.
Syringe 1
1 cc SkinVive (HA)
+ 20 units Neurotoxin
= ~1.8-2 cc total
Syringe 2
1 cc SkinVive (HA)
+ 20 units Neurotoxin
= ~1.8-2 cc total
Inject intradermally using the techniques described in Foundations (34 G needle at 15°, 1-cm intervals, or a microneedle injector).
Technique
Intradermal
Interval
3-6 months
Combination Tip: Can be combined with other skin booster treatments in between sessions (PN, PRF, exosomes, peptides) to maintain and enhance results.
Synthetic biostimulatory polymers (PLLA, PCL) combined with Elastic Lab to stimulate collagen and elastin, rebuilding the skin's structural support over a treatment series.
About Elastic Lab: Elastic Lab (3 cc) is sterile water + multiple amino acids. Because it already provides the aqueous volume, PLLA can be mixed directly with it - avoiding the excess volume of a separate water reconstitution.
Standard Reconstitution
Simplified Mix (Recommended)
Alternative Formula
Primary - Topical: In the US, most skin booster injections are not approved; topical application is the recommended route. Apply to the skin and drive in with a device:
Optional - Injectable PLLA (e.g. Sculptra): follow the dilution sequence below.
Final Injectable Volume
Injection Depth: Full-face skin booster. Needle -> dermis. Cannula -> junction of dermis and subdermis.
Regulatory Note: Injectable use is restricted in the US. Confirm local regulations and product indications; topical application with EBD is the recommended default.
Interval
2-4 weeks
Number of Treatments
3-4 sessions
Series Approach: Biostimulation builds collagen and elastin gradually - results develop over the series. Space sessions 2-4 weeks apart for 3-4 treatments.
Polynucleotide (PN/PDRN), exosomes, and skin botox combined to calm acne, improve scars, fade pigment, and brighten dull skin. PN and exosomes drive repair and regeneration; skin botox refines pores and controls oil and texture.
Tissue repair, hydration, regeneration
Cell signaling, brightening, anti-inflammatory
Pore refinement, oil & texture control
Cocktail Formula
Exosome & PN/PDRN - Topical: apply to skin and drive in with device-assisted delivery for enhanced penetration:
Skin Botox - Topical or Intradermal: 20-40 units, delivered topically with the cocktail or by intradermal injection depending on the plan and local regulations.
Dosing Reminder: When mixed into the booster volume, no extra NaCl is needed. For standalone intradermal skin botox, prepare using Method 2 (see Foundations).
Interval
2-4 weeks
Number of Sessions
3-4 sessions
Series Approach: Acne calming, scar remodeling, and brightening develop progressively. Space sessions 2-4 weeks apart for 3-4 sessions.
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